After many years of research, the Innovation Research Group of the Kunming Institute of Botany, Chinese Academy of Sciences has successfully developed a process for the preparation of azadirachtin, an insecticidal active substance, using the technology of degreasing, extraction, and separation, using the Azadirachium seeds as a raw material. The purity of the alfalfa is more than 90%. The successful development of this process marks a breakthrough in the research on the use of the third generation of pesticides in China. Indigenin is a plant-origin biopesticide that kills more than 200 species of insects and aphids, nematodes, bacteria, fungi and viruses. It is the best botanical pesticide known to produce pollution-free pesticides. For a long time, how to extract high-purity azadirachtin from Azadirachta is an ongoing research topic among scientists in various countries. At present, this technology has completed the preparation of 0.3%, 0.5% of two kinds of azadirachtin EC products, and has been approved by the Ministry of Agriculture for pesticide efficacy testing in the field. Experiments have shown that 0.5% azadirachtin has obvious virulence effects on Plutella xylostella and Pieris rapae. It does not pollute the environment and is a promising biopesticide. 0.3% azadirachtin is more effective against Plutella xylostella. A good control effect, and the effect is positively correlated with the dose, and the best control with high dose (150 ml/mu). After an in-depth study of the availability of pharmaceuticals, it was demonstrated that the control efficacy of azadirachtin EC, the immediate availability of pharmaceuticals, and the use of chemically synthesized pesticides could not be replaced in terms of environmental protection and reduction of pesticide residues.