Contamination and rapid quantitative detection of aflatoxin M1 in milk and dairy products - accurate quantification in -8 min

I. Overview of aflatoxin M1

Aflatoxin M1 (AFM1) is a hydroxylated metabolite of aflatoxin B1, first discovered by Alleroft in 1963 and named aflatoxin M1 in 1965. AFM1 is mainly found in the edible parts of animals, such as liver, muscle, blood, etc. It can also be excreted through urine and milk. Aflatoxin M1 is very toxic, and the intake of dairy products can cause great harm to the human body. Its melting point is quite high at 299 °C, producing blue-violet fluorescence at 365 nm ultraviolet light; its physical and chemical properties are very stable, and the milk is pasteurized and hardly destroyed.

AFM1 is highly carcinogenic and genetically mutated. After entering the human body, AFM1 has different degrees of damage to the blood, liver, kidneys and muscles, among which the liver and kidney are the most harmful. When combined with hepatitis B virus in the liver, a multiplicative risk effect is formed, leading to liver cancer. In addition, when AFM1 is contained in infants' milk and breast milk, it will have a great impact on infant health, which may cause developmental delay, decreased renal function, early onset of hepatocellular carcinoma and may even lead to acute poisoning.

Second, aflatoxin M1 national limit standard

In recent years, countries around the world have developed a limited number of AFM1 standards for milk and dairy products.

Dairy and dairy products are increasingly consumed in China, and the variety is becoming more diverse. At present, with a series of AFM1 pollution incidents reported, people's understanding of it has gradually deepened. AFM1 The most important way to harm humans is through the intake of milk and dairy products, so the pollution control of AFM1 in milk and dairy products is extremely important. In order to ensure the safety of goods in the dairy market, the rapid detection of AFM1 in dairy products is particularly important.

Third, Shanghai fly test biological milk and dairy products aflatoxin M1 rapid quantitative detection program - accurate quantification of -8min

Based on the leading fluorescence quantitative FPOCT technology platform, Shanghai Festo Biotechnology is the first to introduce aflatoxin M1 fluorescence quantitative rapid detection system, including aflatoxin M1 detector and aflatoxin M1 fluorescence quantitative rapid test strip, which can be fast in 8min. Accurate and quantitative detection of the residual content of aflatoxin M1 in milk and dairy products, the sample preparation is simple, the detection operation is simple, the result is accurate and reliable, and can be printed on site. The accuracy is consistent with the detection result of HPLC method, and is suitable for all kinds of milk and Dairy processing companies, third-party testing agencies and government regulatory agencies.

3.1. Aflatoxin M1 Fluorescence Quantitative Rapid Detection System Performance

Detection sensitivity: 0.025μg/kg;

Quantitative linear range: 0.05μg/kg - 1μg/kg;

Detection time: 8min;

Accuracy: recovery rate is 80%-125%;

Specificity: no cross-reactivity with other mycotoxins at a concentration of 1000 μg/kg;

3.2. Detection operation process

1, dilution;

2. Adding reaction (8min);

3, reading, print test report;

3.3. Results interpretation and output

Reading with a portable aflatoxin M1 detector makes the test results more accurate and objective, avoiding human error.

The test result will be presented on the liquid crystal display of the fluorescence reader. At the same time, the paper test report can be obtained by pressing the print button. In addition, after the WIFI data upload function of the instrument is turned on, the relevant data information will be automatically uploaded to the “Food Safety Traceability Management”. Cloud platform for easy traceability and quality management.