"Science" published non-invasive micro-test technology to study the results of Ca2+ flow rate

March 17, 2011, research professor of the University of Lisbon, Portugal, José Feijó in the world-renowned magazine "Science" to "Research Article" The form was published online, and Professor Liu Laihua from the College of Resources and Environment of China Agricultural University participated in the study. The increase of intracellular free Ca ²⁺ constitutes the basic signal transduction mechanism of eukaryotic cells, but how the Ca ²⁺ channel protein initiates the signal has been controversial.

Scientists use NMT technology (vibrating microelectrodes or NMT) in this study was measured after Ca ²⁺ flow Tube amino acid stimulation, direct evidence in plants and animals having similar neurotransmitter system. Glutamate receptors found similar gene (GLRs) ²⁺ influx by reducing the plasma membrane Ca, thereby regulating the pollen tube to the top of cytosolic Ca ²⁺ in a concentration gradient, and ultimately affect the morphology and pollen tube growth built. In addition, knockdown of the pollen tube serine racemase (a mutant of SR1) decreased the activity of GLRs, resulting in defects in growth. This study reveals a novel signal transduction mechanism between amino acid-regulated male gametophytes and pistil tissue, similar to the common mechanisms of the animal's nervous system. This is not only a major discovery of botany, but also a great reference for research in other fields such as zoology and medicine.

Non-invasive micro-testing technology (NMT, ) is a living-study technology that can acquire the flow velocity of ions and small molecules in real time without labeling. It is the best way to study the biological signal transduction and neurotransmission system. one. José Feijó Laboratories has published several papers in high-level journals using non-invasive micro-testing techniques in recent years. The paper published this time is representative of the research work of the laboratory, and it also marks that the application of non-invasive micro-test technology is more and more extensive, and the results produced are more and more influential.

Keywords: Similar glutamate receptor gene (Glutamate Receptor-Like Genes, GLRs ); calcium channel (Ca ²⁺ Channel); pollen tube (Pollen Tube); D-type serine (D-Ser)

References:. Michard E et al Science, DOI: 10.1126 / science.1201101

Link: http:// science.1201101