Cell culture equipment selection: In terms of size, cell culture flasks can be divided into about 600ml, 250ml, 50ml and 25ml, which are generally surface-modified, suitable for cell attachment and growth. 600ml and 300ml are mostly used for large-scale culture (such as culture of monoclonal cells), and 50ml is used for general cell experiments (general passage, preservation of cells, cells for experiments, etc.), 25ml is generally used. To resuscitate cells or cells with less cells, and to make multiple cells when making primary cells to avoid cross-contamination. Of course, there are other things, such as a circle, which can be chosen according to personal preferences and experiments.
Depending on the material used to make the cell culture bottle, there is also a distinction between glass and plastic. Here is a little bit of your own opinion. Different cells have different sensitivity to pancreatic enzymes, so in the experiment we often encounter some cells that have not digested for a long time, and the cell state has been declining until the final death and the progress of the experiment is not smooth. Of course, sometimes it can be achieved by adding some EDTA, etc. to enhance the ability of pancreatin, or to increase the concentration of trypsin. In the process of culturing some cells, I found that some cells have different adhesion to glass and plastic products, such as RAW264.7, smooth muscle cells, etc., after converting the culture flask, it can be found that it is well digested, and it does not change its adherence ability when returning to the culture bottle of the original product. Here I hope that experienced comrades can share your experience.
As for the purchase of the culture plate, there is actually no experience. What I want to say is to avoid buying domestically produced culture plates. Some domestically produced culture bottles are OK, but the culture plates we bought a lot of domestically produced, the effect is not very good, the cells are The inside is basically very very embarrassing. Therefore, if you have enough money, you can buy these things if you can buy them. The basics of big companies can be bought, BD, Corning and so on. These things are in principle one-off, but most of our labs simply can't do it. In fact, soaking in acid, washing and reuse is no problem. After all, we don't have the wealth of foreigners, so we are tired, more Do some physical exercise and exercise your body.
There are many brands of cell culture consumables, the best are Corning Costar, Nunc and BD Falcon; better German Greiner and Swiss TPP, the two main disadvantages of the two brands are the single product category. And the visibility is not enough, and there may be some gaps in the quality; then there are some miscellaneous brands such as Orange, no matter the quality or popularity.
Because the country is currently mainly the world of Corning Costar, Nunc and Falcon, let's talk about their respective situations:
Depending on the material used to make the cell culture bottle, there is also a distinction between glass and plastic. Here is a little bit of your own opinion. Different cells have different sensitivity to pancreatic enzymes, so in the experiment we often encounter some cells that have not digested for a long time, and the cell state has been declining until the final death and the progress of the experiment is not smooth. Of course, sometimes it can be achieved by adding some EDTA, etc. to enhance the ability of pancreatin, or to increase the concentration of trypsin. In the process of culturing some cells, I found that some cells have different adhesion to glass and plastic products, such as RAW264.7, smooth muscle cells, etc., after converting the culture flask, it can be found that it is well digested, and it does not change its adherence ability when returning to the culture bottle of the original product. Here I hope that experienced comrades can share your experience.
As for the purchase of the culture plate, there is actually no experience. What I want to say is to avoid buying domestically produced culture plates. Some domestically produced culture bottles are OK, but the culture plates we bought a lot of domestically produced, the effect is not very good, the cells are The inside is basically very very embarrassing. Therefore, if you have enough money, you can buy these things if you can buy them. The basics of big companies can be bought, BD, Corning and so on. These things are in principle one-off, but most of our labs simply can't do it. In fact, soaking in acid, washing and reuse is no problem. After all, we don't have the wealth of foreigners, so we are tired, more Do some physical exercise and exercise your body.
There are many brands of cell culture consumables, the best are Corning Costar, Nunc and BD Falcon; better German Greiner and Swiss TPP, the two main disadvantages of the two brands are the single product category. And the visibility is not enough, and there may be some gaps in the quality; then there are some miscellaneous brands such as Orange, no matter the quality or popularity.
Because the country is currently mainly the world of Corning Costar, Nunc and Falcon, let's talk about their respective situations:
Corning Costar
This is originally two companies, Corning is a Fortune 500 company in the United States, there are a bunch of business, involving fiber, glass, ceramics and other fields. Its Life Science division is a series of laboratory products such as cell culture consumables, shifters, and filtration; later Corning acquired Costar's cell culture consumables, collectively known as Corning Costar.
Corning (Costar) quality is the best of its kind, almost never heard of its quality; its product line is also full, and some brands have almost all of it (except BD IVF and BioCoat, and Nunc There are several products in the multi-layer culture bottle), and it also has some unique features, such as Costar's Transwell, Corning plastic reagent bottles and filters. In addition, Corning has the longest time in the country, with a high reputation and a good customer base.
This is originally two companies, Corning is a Fortune 500 company in the United States, there are a bunch of business, involving fiber, glass, ceramics and other fields. Its Life Science division is a series of laboratory products such as cell culture consumables, shifters, and filtration; later Corning acquired Costar's cell culture consumables, collectively known as Corning Costar.
Corning (Costar) quality is the best of its kind, almost never heard of its quality; its product line is also full, and some brands have almost all of it (except BD IVF and BioCoat, and Nunc There are several products in the multi-layer culture bottle), and it also has some unique features, such as Costar's Transwell, Corning plastic reagent bottles and filters. In addition, Corning has the longest time in the country, with a high reputation and a good customer base.
Nunc
Nunc, a Danish brand. In 1995, when I merged with Nalgene, Nalgene must have heard that almost every new laboratory will buy Nalgene. When you sell Epp centrifuges, you will often encounter teachers who ask for large centrifuge tubes. Nalgene's. The first two years in China have done a good job, but it seems to be not very good now. It is estimated that the price is not coming down. There is also a problem with the distributor. The agent is now Invitrogen. It seems that the distributors are messed up. These distributions The business is not good, the high price that sells out of the chaos, and the price can not be sold. However, Nunc is still a very good brand, the product is very comprehensive, and its ELISA plate is very advantageous, Falcon and Corning are not comparable, it is estimated that only Greiner and it have a fight. The reason why I am not doing well now is mainly the agent.
Nunc, a Danish brand. In 1995, when I merged with Nalgene, Nalgene must have heard that almost every new laboratory will buy Nalgene. When you sell Epp centrifuges, you will often encounter teachers who ask for large centrifuge tubes. Nalgene's. The first two years in China have done a good job, but it seems to be not very good now. It is estimated that the price is not coming down. There is also a problem with the distributor. The agent is now Invitrogen. It seems that the distributors are messed up. These distributions The business is not good, the high price that sells out of the chaos, and the price can not be sold. However, Nunc is still a very good brand, the product is very comprehensive, and its ELISA plate is very advantageous, Falcon and Corning are not comparable, it is estimated that only Greiner and it have a fight. The reason why I am not doing well now is mainly the agent.
BD Falcon
Falcon should be called Discovery Labware. The supplier of Labware is the BD company in the United States. BD is no different than the first two Corning and Apogent, a century-old store, has entered the top 500. BD Bioscience (the life sciences part of BD) has several products: Pharmingen, Clontech and Discovery Labware. Discovery Labware was originally only Falcon, and later added Biocoat. Some time ago, BD acquired Genetest, which is also under Labware, but the most important one is Falcon. According to BD, Faclon is the earliest brand in this field, with the highest foreign share, and can sell hundreds of millions of dollars a year. Corning is nothing but a latecomer. In addition, BD has a very good set in the treatment of culture vessels. According to her technicians, these culture vessels are treated in a closed environment and the surface treatment is more uniform; other brands are processed in an open environment, It's a bit worse; in addition, Falcon's packaging is good, it is double-layered, and some middlemen will choose Falcon for this, because such packaging is suitable for long-distance transportation.
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