Garlic is like wet and fertile and cold-tolerant, and the loamy soil and sandy loam soil with deep soil layer, loose structure and good drainage are most suitable for planting. The main roots of garlic are concentrated in the soil layer of 5 to 25 cm. It is not suitable for continuous cropping and can be rotated with corn and rice.
Fertilizer rule
Autumn sowing garlic has a long growth period, generally 240 to 260 days. After the differentiation of buds and flower buds, it is the peak period of nitrogen, phosphorus and potassium nutrient uptake in garlic. In this period, the leaves are flourishing, the growth of the moss head is fast, the yield is high, and the fertilizer needs a large amount of water. Through the test, each 100 kg of garlic needs to absorb 1.42 kg of nitrogen, 0.44 kg of phosphorus and 0.99 kg of potassium. The amount and proportion of nitrogen, phosphorus and potassium nutrients are also very different in different growth stages.
Fertilization technology
Scientific and rational fertilization should be carried out according to the soil fertility level and the demand of different growth stages of garlic. Grasping the principle of fertilization of “before, light, medium and heavy, then supplementingâ€, it is necessary to prevent the excessive prosperous period in the early stage and avoid the early defermentation and premature aging.
The planting of garlic blocks requires deep cultivation and fine soil, which enhances the permeability of the soil, is conducive to the transformation of soil microbes and soil nutrients, improves the storage capacity of the soil, and is conducive to the development of roots and bulb expansion. It is necessary to plough and plough after the harvest of the crops in the early stage, and the depth of cultivation is 20 to 25 cm. Because it is covered with mulch film, it should not be topdressed during the growth of garlic. Therefore, before planting, it should be combined with the ground preparation to apply the base fertilizer once, increase the application of farmyard manure 2500-3000 kg per mu, 40-75 kg of chlorine-based compound fertilizer, ferrous sulfate. , manganese sulfate, borax, zinc sulfate, copper sulfate each 1.5 to 2 kg.
Heavy application of joint fertilizer. Garlic is different from other crops. When the climate is normal, the garlic in the greening period is generally not managed in the field to avoid the occurrence of bacterial soft rot caused by the sudden drop of ground temperature caused by watering too much, so that the growth of garlic It is affected, so the first top dressing should be carried out at the jointing stage, and the nitro compound fertilizer can be 20 to 25 kg. After 15 to 20 days, the second fertilizer will be chased, and the application amount per mu will be about 20 kg.
Skillfully applied to differentiate the pumping fertilizer. When garlic enters the stage of differentiation and twitching, vegetative growth and reproductive growth are carried out simultaneously, and the demand for phosphorus is strong. It is also the most critical top dressing in garlic. It must wait until the garlic stalks (now tail) are chasing high-phosphorus high-potassium compound fertilizer 15~ 20 kg, first dissolved and then applied.
Top dressing. Medium and trace elements such as calcium, magnesium, iron, manganese, zinc, copper, etc. are all necessary for the growth and development of garlic. They can be supplemented by foliar spray fertilizer, or combined with pest control, but It is necessary to pay attention to the similar properties of medicines and fertilizers. Spraying time: It should be done before 11 am or after 4 pm on a sunny day. Type of spray fertilizer: use 2 kg of ordinary calcium or 1 kg of sulfur-based compound fertilizer, soak for 50 hours with 50 kg of water, and spray 1 c of its clear liquid. It is also possible to use a potassium dihydrogen phosphate 0.2% to 0.4% solution and a 0.15% manganese sulfate, 0.5% borax, 0.2% zinc sulfate, and 0.15% copper sulfate solution. In the middle and late stages of garlic growth, according to the growth and appearance, 1 to 2 foliar sprays can be carried out, once every 7 to 10 days.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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