l. Variety selection
Broccoli belongs to the likes of cold vegetables, and the general varieties can be cultivated in the greenhouse in autumn and winter. However, in order to improve economic efficiency, it is necessary to pay attention to the combination of early, mid-late and late-maturing varieties, and choose varieties with strong plant growth, dark green leaves, waxy, tight flower buds, small buds and large balls. According to the test: Oriental Green Treasure, Yuguan, Li Green, and Fangluo are early-maturing varieties, Green Ridge, Green Show, and Oriental Green are moderately late-maturing varieties, and they can be cultivated in autumn and winter in the greenhouse.
2. Nursery
Broccoli's seedling age is short (30 to 35 days), depending on the specific situation, it can be raised in the middle and late October, and will be listed in large quantities before the Spring Festival. Seedlings can be used with nutrient meal (10 cm 10 cm) or nutrient grid method (8 cm 8 cm). The nutrient soil is configured with 8 parts of fertile garden soil plus 2 parts of fully decomposed chicken manure or sheep manure, double-grained on-demand, and used in mu The amount is 20 grams and the seeding depth is 0.5 to 1 cm. After sowing, the water is poured once, and the weeds are removed after emergence in 2 to 3 days. The seedbed should be kept moist frequently. When 1 to 2 true leaves and time seedlings are taken 1 or 2 times, the weak and over-milk seedlings are removed. The bed temperature is kept at 20-30 ° C. On the contrary, if the bed temperature is too low and the seedling age is too long, it will cause small old seedlings, resulting in short plant shape and reduced yield after planting. If the bed temperature is too high and the temperature is too high, the weak seedlings will be formed, resulting in weak plant growth and reduced yield. When the seedlings grow to 5 to 7 true leaves for about 30 to 35 days, the fertilizer is controlled, the water is controlled, the ventilation is dehumidified, and the seedlings are incubated for 3 to 5 days to increase the survival rate of the transplanting.
3. Colonization
Before planting, the application of high-quality farmyard manure 4000 kg, diammonium 15 kg, ammonium nitrate 10 kg mixed tillage, fine and flat, ridges are planted with 70 plus 50 cm wide and narrow rows, that is, the width of the ridge is 70 cm, the width of the furrow is 50. Centimeter, the ridge surface is covered with 90 cm mulch, the plant spacing is 40 cm, and 2,750 mu seedlings are planted. After planting, the fertilizer and water are attacked in the same way, and the temperature is maintained at 8 to 24 °C. The plant growth is promoted as soon as possible, and the leaf area is increased. About 10 days, with the watering and topdressing 15 kg of ammonium nitrate, the top flower ball appeared before the application of ammonium nitrate 6 kg, the temperature after the flower ball was maintained at 15 ~ 18 ° C, less than 8 ° C, the flower ball grows slowly, greater than 24 °C, the flower ball is loose, and the whole plant growth temperature should not exceed 32 °C.
4. Harvest
When the flower ball grows to 0.4-0.6 kg, the diameter is 12-18 cm, the color is dark green, the flower ball is tight, and it is harvested when it is not loose. On the contrary, if the harvest is too early, the yield is low, the quality is degraded, and when it is too late, the moss is flowering and the edible value is lost.
5. Pest Control.
The seedlings are sick. Seed dressing or soil treatment with 0.2% 40% seed dressing, if necessary, spray 25% toxic mildew WP, 0.2% to 0.3% 75% chlorothalonil WP or 70% dysen Manganese zinc powder seed dressing.
Downy mildew. After the discovery of the central diseased plant, 72% of Kelu wettable powder 800 times solution, 58% of metalaxyl manganese-zinc wettable powder 500 times solution, and 69% of Ankang Mn-Zn 1000 times solution can be used. The drug was stopped 7 days before harvesting.
Soft rot. Avoid continuous cropping with Solanaceae melons and other cruciferous vegetables. Under the membrane, dark irrigation is carried out to control pests and reduce wounds. Seeding with 1% to 1.5% of the seed amount of agricultural anti-751, spraying streptomycin sulfate or 72% agricultural streptomycin soluble powder 3000-4000 times solution, once every 10 days, continuous prevention 2 to 3 times, mining Stop taking the medicine 3 days before the reception.
Pieris rapae and aphids. 50% anti-Pu Wei WP 2000 times solution and 25% Oxygen sinus 1000 times solution sprayed against insects, 2 to 3 times during the whole growth period, and the interval between 2 doses should not be less than 7 days. The drug was stopped 7 days before the harvest.
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[Sample requirements]
The collected nasopharyngeal swab samples should be transported at 2°C to 8°C and sent for inspection immediately, and the sample delivery and storage time should not exceed 48 hours.
[Testing method]
1. Before sampling, mark the relevant sample information on the label of the sampling tube.
2. According to different sampling requirements, use a sampling swab to sample in the nasopharynx.
3. The specific sampling methods are as follows:
a) Nasal swab: Gently insert the swab head into the nasal palate, stay for a while and then slowly turn to exit. Wipe the other nostril with another swab, immerse the swab head in the sampling solution, and discard the tail.
b) Pharyngeal swab: Wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with a swab, also immerse the swab head in the sampling solution, and discard the tail.
4. Quickly put the swab into the sampling tube.
5. Break the part of the sampling swab higher than the sampling tube, and tighten the tube cover.
6. Freshly collected clinical specimens should be transported to the laboratory within 48 hours at 2°C to 8°C.
[Explanation of test results]
After the sample is collected, the sampling solution turns slightly yellow, which will not affect the nucleic acid test result.
[Limitations of the test method]
1. For samples that are seriously contaminated due to improper storage after collection, the final test results will be affected.
2. If the sample is not stored at the specified temperature, the final test result will be affected.
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