Fermentation tank machinery operation method

2, sterilization

At least two people are required to be present during sterilization, one person is responsible for operating various valves, and the other is responsible for observing the reported parameters.

After confirming that each valve is in the closed state, open the steam generator inlet valve, start the steam generator, and the steam generator starts to automatically enter the water. At this time, open the drain valve in the lower part of the steam generator, try to put the water rust and close the drain valve. . Pay attention to the water level in the steam generator to prevent accidents. At this point, fully open the jacket outlet valve, slowly open the steam generator main valve, slowly open the jacket intake valve, and start to pass water vapor to the jacket. At the beginning, the steam pressure may be unstable, the pressure gauge pointer swings violently, and the small jacket intake valve can be properly closed, and then the sampling valve outlet steam valve is opened, the sampling valve is introduced into the steam valve, the bottom valve is discharged from the steam valve, and the bottom valve is introduced into the steam valve. , sterilize the sampling valve and the bottom valve, and discharge the water vapor with unstable pressure. When the water vapor pressure does not change drastically, the valve is finally closed and the bottom valve is released. The temperature inside the tank gradually rises. When the temperature is set to 90°, the stirring motor stops rotating. At this time, the tank main intake valve is opened, the tank outlet valve is opened, and the following valves are opened in sequence: the outlet regulating valve is opened, and the tank is slowly opened. The steam inlet valve introduces steam into the tank, and the pressure of the air filter begins to rise. When the pressure exceeds 0.1MP, the condensate valve is opened to drain the water, and the large tank is continuously opened into the steam valve. After the air in the tank is removed, the pressure inside the tank slowly rises and adjusts. The outlet valve is adjusted to the temperature required for sterilization, and the timing begins when the sterilization temperature is reached. Adjust the jacket intake valve, the tank inlet steam valve and the outlet regulating valve to maintain the temperature and tank pressure at the set temperature. After the sterilization time is reached, the opened inlet valves are closed in turn, the steam main valve is closed, the exhaust gas exhaust valve in the tank is completely released, the pressure in the tank is released, and the steam generator power is turned off. Open air compressors, tanks and the like when the pressure dropped to 0.05MP, slowly open the intake control valve, a positive pressure is maintained within the fermentation tank.

During the sterilization process, the operator should wear long trousers with protective gloves to prevent burns. If the steam generator automatically enters the water during the sterilization process, the total steam pressure will drop rapidly. If necessary, the steam main valve can be opened appropriately to maintain the pressure, but the pressure change should be observed carefully. When the steam is passed through the tank, the tank pressure drops rapidly after sterilization, paying attention to the timing of the sterile air. Try not to operate the air compressor and steam generator at the same time. The air filter pressure should not exceed 0.2 MP during sterilization. The size of the condensate will affect the concentration of the liquid in the tank after sterilization, taking into account the increase in steam condensate.

3. Fermentation

Controlling the size of the intake valve exhaust valve can adjust the ventilation volume and tank pressure, and control the stirring rotation number to make the fermentation dissolved oxygen meet the process requirements. When the fermentation is started, the tank inlet water inlet valve is opened, and the jacket outlet valve and the jacket inlet valve are opened. At this time, the system automatically controls the fermentation temperature by controlling the jacket water temperature. Due to the positive pressure inside the tank, close the small intake valve when inoculation, properly reduce the tank pressure to 0.01-0.02MP, use the flame ring protection to open the inoculation port quickly (to prevent the airflow from extinguishing the flame), and quickly close the inoculating port after inoculation. After sterilizing each bottle and accessory hose of the feeding system, connect it to the main machine (pay attention to the direction), puncture the silicone pad under the protection of the flame ring, and install the feeding system. At this point, the real-time monitoring of each parameter of the system should be displayed on the workstation. Pay attention to the parameter recording work.

When the fermentation is carried out to the later stage, the bacteria may block the gas outlet, and the phenomenon is that the ventilation volume is decreased and the tank pressure is slightly increased. At this time, the air outlet valve is rotated left and right to relieve the blockage.