Some customers' centrifuges sometimes have some small accidents during use, mainly due to the misunderstanding of the maintenance and repair of the centrifuge.
For example, the rotor can be broken due to metal fatigue, elastic deformation beyond the limit, overspeed, overstress, chemical corrosion, improper selection, use of rotor imbalance and temperature loss control, sample leakage, sample leakage, rotor damage . For the above situation, the operator is required to be proficient in the operation procedure, correctly select the appropriate centrifuge tube and centrifugal rotor, pay attention to strictly control each step of operation, minimize unnecessary damage caused by human beings, and use it in the safety factor and warranty period of the rotor. . Therefore, our company has an ideal accident for all products, this test is currently very valuable in the same industry in China.
Unstable power supply is one of the reasons for the start of the freezer and the poor cooling effect. The same as the failure of the motor, the power supply and fuse should be checked separately. The voltage is too low, the safety device failure can also make the freezer unable to start, the voltage is too low may be the grid voltage is low; the distribution board wiring is too much; the power line is too long (ideal length is 3 meters). The power line capacity is not enough, which not only reduces the voltage, but also causes accidents. When the power supply voltage drops to 180-190VJ, the freezer cannot start, which affects the cooling effect. The ventilation performance is not good. If the instrument is installed in direct sunlight or poor ventilation, the radiator is not effective, or the radiator is covered with dust, which will affect the cooling effect. The weight of the centrifuge tube is unbalanced and placed asymmetrically; there is foreign matter in the rotor hole to make the load unbalanced; the fixed nut on the upper end of the shaft is loose, the shaft is rubbed or bent; the rotor of the motor does not generate noise in the center of the magnetic field; the damage of the rotor itself can cause the body The vibration is severe and the noise is abnormal. Most of the above reasons are caused by incorrect operation. After finding out the cause, correct operation can eliminate the abnormal phenomenon. In the course of work, if any abnormality occurs, it should be stopped immediately, and it must not be forced to run, so as to avoid unnecessary losses.
The following is a brief introduction to the maintenance and precautions of the centrifuge:
The installation of the centrifuge should be based on the performance of the centrifuge. The general low-speed centrifuge (desktop) can be placed on a stable and sturdy table (the base is equipped with rubber feet, with the help of atmospheric pressure and The weight of the instrument itself is close to the table top; large-capacity low-speed centrifuges and high-speed centrifuges and high-speed refrigerated centrifuges should be placed on a solid floor and placed horizontally. The ultracentrifuge weighs several hundred kilograms and should be placed on a very solid ground with dustproof and moisture-proof equipment to ensure that the centrifugal chamber reaches a certain degree of vacuum and normal operation. Before selecting a suitable rotor centrifuge, the load should be balanced. The smaller the weight error, the better. Otherwise, it will cause severe vibration and damage the centrifugal rotor and the shaft. The centrifuge bracket centrifuge separation bracket must be placed after the centrifuge tube is operated, and the empty frame operation is strictly prohibited. When the centrifuge is started, the speed switch or G value knob is placed in the low gear, and then gradually adjusted to the desired value after starting. After one work, turn the knob to the low gear to work again, and prohibit the direct start at high speed or G gear.
The high rotational speed of the rotor is to reduce the operating time of the rotor and the number of times of operation of the rotor to a predetermined life. When the rotor is subjected to local surface damage or slight corrosion in the tube hole. When using stainless steel centrifuge tubes, bushings, thick wall tubes or centrifuge bottles. When the average density of the sample exceeds 1.2 g/cm3, the practical rotational speed N is: where N is the practical rotational speed and N is the large rotational speed of zui. When the rotor is exposed to high temperatures. The maintenance rotor of the rotor should be strictly checked for foreign matter and dirt in the hole before each use to maintain balance. The rotor must be kept dry and clean and must not be scratched. If you accidentally lose it, you should check the rotor for X-ray film and confirm that there is no internal damage. Centrifuge Tubes Centrifuge tubes can be made from a variety of different materials such as polyethylene (PE), cellulose (CAB), polycarbonate (PC), polypropylene (PP), and the like. The tensile strength and elongation of various centrifuge tubes are different and should be selected as needed. Pay attention to ensure that the sample, solvent and gradient materials do not corrode the centrifuge tube and cap assembly. The centrifuge tube that has expired, aged, cracked or corroded is not used or slowed down to control the number of times the plastic centrifuge tube is used. Pay attention to the specifications. The centrifuge chamber cover has to be opened during centrifugation, and the rotating rotor and the centrifuge tube must not be hit by hand or foreign matter. The sample should be pre-cooled for 2000 r/min for a certain period of time. The pre-cooling control temperature is about 0 °C. The rotor can also be pre-cooled for several hours in the refrigerator. The centrifuge cup can be stored directly in the refrigerator. cold.
Abnormal phenomena during centrifugation During the centrifugation process, if abnormal noise and vibration are found, the machine is immediately shut down for inspection. It is not allowed to continue operation until the cause is found. The drive part bearing should be cleaned and coated with high-speed grease every 1500 hours or so. The joint between the shaft and the rotor should be greased and rust-proof. If it is not used for a long time, it should be coated with anti-rust oil and oil paper. When not in use, it should be turned on 1-2 times a month at low speed, half an hour each time to ensure the normal operation of all parts. As mentioned above, there are many types of centrifugal rotors, and the shapes are different. It is troublesome to convert the centrifugal radius between each other. For this reason, the K-factor method of the centrifugal rotor is proposed, which simplifies the conversion between various centrifugal rotors.
The sink coefficient is differentiated, S = dlnxl / w2t. Usually centrifugal sedimentation from zui small radius a to zui large radius b, then S = In (b / a) / W2dt, set 1n (b / a) / W2 = K, T = dt get S = K / T
From the above formula, the K value of each rotor at different rotational speeds can be calculated and listed as a K value table. If you need to change the centrifugal speed during the experiment, just find the K value of the corresponding speed, and then according to S=K/T, you can calculate the time that should be centrifuged after changing the speed. For example, if the original speed is K, the value is K. The centrifugation time T1, now to use another speed of centrifugation, only need to find the corresponding K value from the table, such as K2 because the sample sedimentation coefficient S is certain, so there is S = K1/T1 = K2 / T2 can Calculating T2 at K2, it can be seen from the above equation that the K value of the rotor of the same type reflects the centrifugal ability of the rotor. The smaller the K value, the larger the centrifugal capacity, the shorter the centrifugal time, and the higher the rotor use value.
In short, the centrifuge is a basic equipment in the laboratory, and there are many high-rise buildings on the ground, no matter how precise the test. It is necessary to complete the Zui-based process through these basic equipment.
For example, the rotor can be broken due to metal fatigue, elastic deformation beyond the limit, overspeed, overstress, chemical corrosion, improper selection, use of rotor imbalance and temperature loss control, sample leakage, sample leakage, rotor damage . For the above situation, the operator is required to be proficient in the operation procedure, correctly select the appropriate centrifuge tube and centrifugal rotor, pay attention to strictly control each step of operation, minimize unnecessary damage caused by human beings, and use it in the safety factor and warranty period of the rotor. . Therefore, our company has an ideal accident for all products, this test is currently very valuable in the same industry in China.
Unstable power supply is one of the reasons for the start of the freezer and the poor cooling effect. The same as the failure of the motor, the power supply and fuse should be checked separately. The voltage is too low, the safety device failure can also make the freezer unable to start, the voltage is too low may be the grid voltage is low; the distribution board wiring is too much; the power line is too long (ideal length is 3 meters). The power line capacity is not enough, which not only reduces the voltage, but also causes accidents. When the power supply voltage drops to 180-190VJ, the freezer cannot start, which affects the cooling effect. The ventilation performance is not good. If the instrument is installed in direct sunlight or poor ventilation, the radiator is not effective, or the radiator is covered with dust, which will affect the cooling effect. The weight of the centrifuge tube is unbalanced and placed asymmetrically; there is foreign matter in the rotor hole to make the load unbalanced; the fixed nut on the upper end of the shaft is loose, the shaft is rubbed or bent; the rotor of the motor does not generate noise in the center of the magnetic field; the damage of the rotor itself can cause the body The vibration is severe and the noise is abnormal. Most of the above reasons are caused by incorrect operation. After finding out the cause, correct operation can eliminate the abnormal phenomenon. In the course of work, if any abnormality occurs, it should be stopped immediately, and it must not be forced to run, so as to avoid unnecessary losses.
The following is a brief introduction to the maintenance and precautions of the centrifuge:
The installation of the centrifuge should be based on the performance of the centrifuge. The general low-speed centrifuge (desktop) can be placed on a stable and sturdy table (the base is equipped with rubber feet, with the help of atmospheric pressure and The weight of the instrument itself is close to the table top; large-capacity low-speed centrifuges and high-speed centrifuges and high-speed refrigerated centrifuges should be placed on a solid floor and placed horizontally. The ultracentrifuge weighs several hundred kilograms and should be placed on a very solid ground with dustproof and moisture-proof equipment to ensure that the centrifugal chamber reaches a certain degree of vacuum and normal operation. Before selecting a suitable rotor centrifuge, the load should be balanced. The smaller the weight error, the better. Otherwise, it will cause severe vibration and damage the centrifugal rotor and the shaft. The centrifuge bracket centrifuge separation bracket must be placed after the centrifuge tube is operated, and the empty frame operation is strictly prohibited. When the centrifuge is started, the speed switch or G value knob is placed in the low gear, and then gradually adjusted to the desired value after starting. After one work, turn the knob to the low gear to work again, and prohibit the direct start at high speed or G gear.
The high rotational speed of the rotor is to reduce the operating time of the rotor and the number of times of operation of the rotor to a predetermined life. When the rotor is subjected to local surface damage or slight corrosion in the tube hole. When using stainless steel centrifuge tubes, bushings, thick wall tubes or centrifuge bottles. When the average density of the sample exceeds 1.2 g/cm3, the practical rotational speed N is: where N is the practical rotational speed and N is the large rotational speed of zui. When the rotor is exposed to high temperatures. The maintenance rotor of the rotor should be strictly checked for foreign matter and dirt in the hole before each use to maintain balance. The rotor must be kept dry and clean and must not be scratched. If you accidentally lose it, you should check the rotor for X-ray film and confirm that there is no internal damage. Centrifuge Tubes Centrifuge tubes can be made from a variety of different materials such as polyethylene (PE), cellulose (CAB), polycarbonate (PC), polypropylene (PP), and the like. The tensile strength and elongation of various centrifuge tubes are different and should be selected as needed. Pay attention to ensure that the sample, solvent and gradient materials do not corrode the centrifuge tube and cap assembly. The centrifuge tube that has expired, aged, cracked or corroded is not used or slowed down to control the number of times the plastic centrifuge tube is used. Pay attention to the specifications. The centrifuge chamber cover has to be opened during centrifugation, and the rotating rotor and the centrifuge tube must not be hit by hand or foreign matter. The sample should be pre-cooled for 2000 r/min for a certain period of time. The pre-cooling control temperature is about 0 °C. The rotor can also be pre-cooled for several hours in the refrigerator. The centrifuge cup can be stored directly in the refrigerator. cold.
Abnormal phenomena during centrifugation During the centrifugation process, if abnormal noise and vibration are found, the machine is immediately shut down for inspection. It is not allowed to continue operation until the cause is found. The drive part bearing should be cleaned and coated with high-speed grease every 1500 hours or so. The joint between the shaft and the rotor should be greased and rust-proof. If it is not used for a long time, it should be coated with anti-rust oil and oil paper. When not in use, it should be turned on 1-2 times a month at low speed, half an hour each time to ensure the normal operation of all parts. As mentioned above, there are many types of centrifugal rotors, and the shapes are different. It is troublesome to convert the centrifugal radius between each other. For this reason, the K-factor method of the centrifugal rotor is proposed, which simplifies the conversion between various centrifugal rotors.
The sink coefficient is differentiated, S = dlnxl / w2t. Usually centrifugal sedimentation from zui small radius a to zui large radius b, then S = In (b / a) / W2dt, set 1n (b / a) / W2 = K, T = dt get S = K / T
From the above formula, the K value of each rotor at different rotational speeds can be calculated and listed as a K value table. If you need to change the centrifugal speed during the experiment, just find the K value of the corresponding speed, and then according to S=K/T, you can calculate the time that should be centrifuged after changing the speed. For example, if the original speed is K, the value is K. The centrifugation time T1, now to use another speed of centrifugation, only need to find the corresponding K value from the table, such as K2 because the sample sedimentation coefficient S is certain, so there is S = K1/T1 = K2 / T2 can Calculating T2 at K2, it can be seen from the above equation that the K value of the rotor of the same type reflects the centrifugal ability of the rotor. The smaller the K value, the larger the centrifugal capacity, the shorter the centrifugal time, and the higher the rotor use value.
In short, the centrifuge is a basic equipment in the laboratory, and there are many high-rise buildings on the ground, no matter how precise the test. It is necessary to complete the Zui-based process through these basic equipment.
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