The fungal diseases of vegetables are very numerous. The symptoms of the same pathogen may be different in different crops or different growth and development stages of the same crop or different environments. On the contrary, different pathogens may cause the same symptoms. Therefore, judging by symptoms alone is not completely reliable, and pathogenic organisms must be further identified before they can be confirmed. Fungi can be used for direct microscopy and post-moisturizing microscopy. Direct microscopy: Use a needle to pick the mold layer on the surface of the rickets, place it on a glass slide with sterile water, and observe with a low magnification microscope. After moisturizing microscopic examination: cut a small section of rickets, after surface disinfection, placed in a glass culture dish moisturizing culture for 24 hours, pick the moldy layer under a microscope, observe the presence of fungi, shape and size, the initial diagnosis of fungal disease. In the process of moisturizing, saprophytic bacteria are often associated and need to be distinguished; spores or fruit bodies are not easily seen on some diseased surfaces, and spores can not be produced even after moisturizing culture, and special isolation and culture are required.
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