first step:
Plugged, back iMark microplate open switch is turned on after iMark microplate reader, after the self-test, the machine according to the prompts, pressing the Enter key after entering 00000 iMark microplate reader to enter the interface.
The second step:
If the test program has been programmed, press Memory Recall to directly call up the program in the store search menu and read the board directly. If the test program needs to be re-edited, press the program settings in the Edit menu to edit the new test program.
third step:
Edit the new program: Press Edit to edit the menu, first enter the program settings, there are several sub-menus that need to be edited:
1. Threshold setting;
2. Report type setting;
3. Standard setting;
4. Test mode setting
5. Enzyme plate layout settings.
Qualitative tests generally require setting thresholds. Quantitation is generally not required; qualitative tests generally do not require the setting of standards, while quantification requires the setting of standards.
the fourth step:
Threshold setting: There are several sub-items in the threshold setting: not used, constant, quality control, formula, ratio, etc.
1. Not used: It is suitable for quantitative test, and there is no threshold when doing quantitative test;
2. Constant: There are two menus for the single threshold and the threshold range. Single threshold, use the decimal point key and numeric keys to input the positive and gray value area right arrow to select the unit, the up and down keys to select the parameter; threshold range: use the decimal point key and numeric keys to input the positive and gray value area right arrow to select the unit, the up and down keys to select parameter.
3. Quality Control: In this setting, you only need to enter the gray zone value in the single threshold.
4. Formula: There are 5 formulas in the machine, move the cursor to the formula option, and enter the formula to modify the parameters three times. The operator can select the formula that matches the formula according to the formula provided on the kit. The K value and gray area in the change are changed as needed. After the modification, press the enter key to save the settings.
5. Ratio: The ratio of the absorbance average to the concentration value of each calibrator.
the fifth step:
Report type setting
There are several sub-items: raw data, absorbance, limits, matrix values, thresholds, curves, concentrations, and differences.
1. Raw Data Report: Refers to uncorrected absorbance. The single wavelength mode refers to the original absorbance; the dual wavelength mode refers to the difference between the detection wavelength and the reference wavelength absorbance.
2. Absorbance: Refers to a blank corrected report. All 96-well absorbance readings were subtracted from the absorbance values ​​of the blank wells.
3. Limits: Yes to provide a positive result. After the blank hole is deducted, the absorbance is displayed between the high and low limits (*), lower than the low value display (-), and higher than the high value display (+).
4. Matrix value: Refers to the qualitative results of the holes provided in the microplate. After the blank hole is deducted, the absorbance between the upper and lower limits is divided into 10 grades, 0 to 9. Above the upper limit display (+), below the lower limit display (-).
5. Threshold: Qualitative result or converted to concentration.
6. Curve: Reflects a series of standard absorbance regression analysis, the iMark type microplate reader can support the following 10 curve types.
7. Concentration: In the case of using the standard curve, the concentration report is still subjected to regression analysis, and the concentration of each sample is calculated using the standard curve to form a report. If a constant threshold is used and the unit is not an "Abs" or ratio threshold, the system automatically calculates the concentration of each well.
8. Difference: The difference report is the difference between the absorbances of two adjacent rows or columns.
The sixth step:
Standard setting
This setting is for quantitative testing, and this setting is not available for qualitative testing.
Standard setting: (1) Setting of standard information, including the number of standards, concentration, unit (2) The standard curve setting includes the setting of the curve type and the setting of the coordinate axis.
Number of standard products: You can set the number of 0-12 standard products, fill in the concentration of the given concentration in the concentration option; there are dozens of units available in the unit option, and select according to the unit of known concentration. Consistent units.
Curve setting: The iMark microplate reader has a variety of curves to choose from for the fitting of the standard; the setting of the axis: in this option, the X and Y axes of the coordinate axis can be linear or nonlinear. Settings.
The seventh step:
Test mode setting: In the optical measurement mode, the machine defaults to a single wavelength. Move the cursor to a single wavelength, æ‘ right arrow, that is, select the key to select dual wavelength, and then click it to become a single wavelength. Move the cursor to the wavelength, and the right arrow is the selection button to select back and forth in the wavelength parameters set in the machine.
Vibration setting: The machine defaults to on, moving the cursor to the on position, the right arrow turns off; then it is back on. At the time: use the number keys to type in the required time, vibration intensity: use the right arrow to select the middle, strong, weak.
Readings: There are two sub-items in the reading settings, namely reading speed and reading mode. Move the cursor to the reading speed option, which has a quick reading and a step-by-step reading. Use the right button to select between the two options. Fast reading single wavelength 6 seconds, dual wavelength 15 seconds; step reading: single wavelength 15 seconds, dual wavelength 30 seconds; move the cursor to the reading mode, there are two kinds of common readings and evaluation readings, right click in two Select back and forth in the options. After the above parameters are selected, press the Enter key to save the modified contents. The fast reading in the reading speed is suitable for the test with a large amount of test; the normal reading in the reading mode is the result of the reading, and the reading is the average of four readings, so that the read data is more accurate.
The eighth step:
The setting of the ELISA plate layout is divided into manual plate and automatic plate.
1, manual row: æ‘ enter key to enter this menu, select manual row, æ‘ enter key.
The default ELISA plate layout of the iMark microplate reader is that the first vertical row is a blank hole, indicated by B, the second vertical row is a standard product indicated by S; the latter is added samples are indicated by X.
2. Typesetting method: You want to set a hole, for example, set the first vertical blank hole to the standard 9 hole, if the upper left corner of the screen is "F". Just move the cursor to the hole and then tap the button labeled STD. Then the screen shows that the hole is now the standard No. 1 hole. In the æ‘ table, the conversion key is changed. "N", input O9 becomes the standard 9 hole. All hole settings can be set in this way.
In the case where the letter in the upper left corner of the screen is "F", the properties of a hole can be set, such as blank (BLK key); standard (STD key); quality control (QC key), sample (SMP key) Positive control (CP key), negative control (CN key), etc.; in the case where the letter in the upper left corner of the screen is "N", the numerical number of the hole can be modified, ranging from 0 to 96. The letters "F" and "N" can be switched back and forth by the toggle button. If multiple tests are used in a row layout, the template can be saved and used directly when used. The number of standard settings must match the number of standard products in the previous standard information. save. Saving method: After the ELISA board layout is completed, press the ENTER key.
3. Automatic arranging: In this interface, the user can define the specific number of each hole type. Based on these numbers, the instrument will automatically arrange the layout.
The ninth step:
Test name setting
This is the setting of your own program name. The iMark microplate reader has a default name. If you want to modify it to make it easier for you to remember, you can use the cursor up button (AZ) and down button (ZA) to select your easy-to-remember letters and conversion keys. /FUNC Can change its case.æ‘ Enter the key to save the modified name. After the above test is basically set up, press the start button on the target to read the board, and then print automatically after reading, no need to print the button.
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