Comparison of GS-Smart Small Automatic Gel Dyeing Instrument and Benchtop Horizontal Decolorization Shaker in CBB Staining Method

The desktop horizontal decolorization shaker is a common instrument in biological laboratories, commonly used for general gel electrophoresis band fixation, Coomassie Brilliant Blue (CBB) stain decolorization, silver nitrate staining, Western Blot, cell culture and radiation. Autoradiography and other experiments. The general desktop horizontal decolorizing shaker mainly controls the swinging speed of the sample in the solution by adjusting the swing frequency of the fixed-width carrier. This is the basic working principle of the instrument and the main function of her in the above experiments.

In the ordinary Coomassie blue staining and decolorization experiment, the desktop horizontal bleaching shaker is a device often used by researchers. Generally, after setting the swing frequency of the working pool, the CBB staining solution and the decolorizing liquid are successively added, so that the working pool of the shaker is continuously swayed, and then the protein gel is placed to fully infiltrate and wash in the swaying liquid, thereby achieving dyeing and discoloration. However, in addition to the adjustable swing frequency of the working pool, the general desktop horizontal decoloring shaker has no other parameters to set. Although some models of the shaker also add timing function, it is impossible to set up the automatic completion of complicated steps. Therefore, the pre-injection, liquid exchange and drainage require manual operation by the experimenter. In addition, the dyeing and decolorization time can only be grasped by the experimenter himself.

Furthermore, the working pool of the desktop horizontal decolorizing shaker is generally exposed, and during the rocking process, it may spill spilled CBB staining solution, decolorizing liquid, and possibly toxic compounds. This is not only easy to cause pollution, but may even cause safety hazards, thereby jeopardizing the health of the experimental personnel.

In contrast, the Dingyuan GS-Smart small automatic gel stainer leads the benchtop horizontal bleaching shaker in terms of function, design and appearance, for example:

1. Intelligent programming function:

GS-Smart has 3 standard dyeing programs built in, and can store 47 custom programs. It can easily realize the steps of liquid inlet, liquid exchange, liquid discharge, timing shaking and waste liquid recovery. It is unattended and allows the experiment to be completed automatically. This will save a lot of time and effort for researchers who are still using the traditional benchtop horizontal bleaching shaker for CBB dyeing and decolorization experiments.

2. Can be closed customizable dyeing pool:

The GS-Smart dyeing tank can be closed to prevent spillage, block volatiles, and to choose from a variety of sizes. Some researchers to achieve "quick" CBB stain bleaching, bleaching or dyeing liquid diet the CBB was heated to boiling and bleaching dyeing process, the high temperature will accelerate the liquid volatile compounds such as methanol and acetic acid, used at this time if the desktop level decolorization Shakers undoubtedly have certain safety hazards.

3. Integrated design of the fuselage and liquid storage bottle:

This design is the first in the world. Compared with similar products on the market, it reduces the number of bottles and cans that are scattered outside, so that the whole machine footprint is equivalent to that of a common desktop horizontal decolorizing shaker . Not only saves lab space, but also beautifies the overall look.

In summary, in the application of CBB dyeing decolorization experiment, Dingshengyuan GS-Smart small automatic gel staining instrument is completely ahead of the desktop horizontal decolorizing shaker in terms of function, design and appearance. In fact, from the beginning of the spring of 2013, GS-Smart was positioned as an instrument for the Coomassie blue dyeing decolorization experiment. Her ultimate mission was to completely replace the desktop horizontal bleaching shaker in the CBB dyeing decolorization experiment. So that all CBB staining is done automatically!

Keywords: shaker , bleaching shaker, horizontal bleaching shaker