IKA is bringing you good news to the little friends who struggled day and night in the biology lab! Heat shock method to transform E. coli, everyone must be familiar with:
Escherichia coli in 0 °C CaCl2 hypotonic solution, the cells expanded into a spherical shape, the DNA in the transformation mixture formed a DN-resistant hydroxy-calcium phosphate complex adhered to the cell surface, and was subjected to thermal shock treatment at 42 ° C for a short time to promote cell absorption. After a few hours of growth on the rich medium, the globular cells recover and divide and proliferate. In the transformed cells, the recombinant gene is expressed and the desired transformants can be selected on a selective medium plate.
Are you suffering from an unsuccessful experiment? The conversion efficiency is low, or the underlying genes are not transferred, do you find the reason? IKA's ICC type thermostat circulator tells you that this problem is probably that the temperature control is not well controlled.-----It may be that your water bath or heating pot does not really reach 42 °C, or they may not be stable at a constant Temperature.....so the naughty genes can't turn in. Also wait for our German IKA thermostat circulator.
Sodium Acetate,Sodium Acetate Anhydrous,Anhydrous Sodium Acetate,Industrial Grade Sodium Acetate Anhydrous
CHANGZHOU GUANGHUI FOOD INGREDIENTS CO.,LTD , https://www.gsweetfood.com