I. Introduction to the quantitative detection kit for mycotoxin fluorescence
Based on the leading fluorescence quantitative FPOCT technology platform, Shanghai Festo Biotechnology has launched a mycotoxin mobile detection platform--the mycotoxin fluorescence quantitative rapid detection kit, which combines the fast colloidal gold, enzyme-linked immunoassay and accurate chromatographic method, which can be used in 8min. Rapid and accurate quantitative determination of mycotoxins in grain, oil and grain feeds, including aflatoxin B1, zearalenone, vomiting toxin, ochratoxin A, fumonisin, T2 toxin, etc., sample preparation is simple ( Only 7min), the six toxins can be extracted at one time, the operation is simple, only one step is needed, no standard is needed, no standard curve is needed, the result is accurate and reliable, and can be printed on site. The accuracy is highly consistent with the detection result of HPLC method. The rapid on-site detection of mycotoxins provides a new technical means for all kinds of raw material storage and storage, grain and oil processing enterprises, feed processing enterprises, aquaculture enterprises and government-related regulatory authorities.
Second, mycotoxins detection items and performance parameters
Third, the testing process
3.1 Sample preparation process
1, crushing;
2. Oscillation extraction (5min);
3. Centrifugation (2min);
3 .2 detection process
1, dilution;
2. Adding reaction (8min);
3, reading, print test report;
3.3 Result judgment and output
The portable mycotoxin fluorescence quantitative detector is used for reading, and the detection result is more accurate and objective.
The test result will be presented on the liquid crystal display of the fluorescence reader. At the same time, the paper test report can be obtained by pressing the print button. In addition, after the WIFI data upload function of the instrument is turned on, the relevant data information will be automatically uploaded to the “Food Safety Traceability Managementâ€. Cloud platform for easy traceability and quality management.
Fourth, Shanghai fly test biomycotoxin fluorescence quantitative rapid detection system advantages
ïµ 8min fast and accurate quantitative detection: the rapid detection of colloidal gold, enzyme-linked immunoassay, accurate detection of chromatographic mass spectrometry, can achieve rapid and accurate quantitative determination of a variety of mycotoxins in 8min;
ïµ Built-in quantitative standard curve: The instrument has built-in standard curve, no need to use standard products, and no standard curve is needed when testing, which not only saves cost, but also avoids the operator's contact with mycotoxins and protects the safety of operators;
ïµ Multi-in-one sample preparation process: The pre-treatment method is uniform, and one kind of extract can detect all kinds of mycotoxins in one extraction, without any pH adjustment of the extract, which greatly improves the convenience and efficiency of detection;
ïµ éš éš : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : It can also be used for on-site inspection of grain collection to ensure the quality of raw material purchases;
ïµEasy operation: low requirements for supporting instruments and testing personnel, operators can be mastered only by short-term training;
Support for the standard, the authoritative verification of the Grain Bureau: based on the industry standards LST6111-2015, LST6112-2015 and LST6113-2015, and through the authority verification of the Grain Bureau and the Academy of Agricultural Sciences;
ïµThe sample has a wide range of applications: it adopts the patented technology of time-resolved fluorescence, has strong resistance to matrix interference, and has wide applicability. It can detect raw grains such as wheat, corn and rice, as well as various feed materials such as alfalfa, bran and DDGS. It can also detect various vegetable oils such as peanut oil and corn oil;
ïµData uploading and traceability management: Fluorescence quantitative analyzer has the function of wireless data uploading. It can customize the corresponding data management and traceability management system according to the customer's needs, and realize the company headquarters to the branch supervisors and superior supervisory departments to the lower-level supervisory departments or The real-time mastery and analysis of the inspection data of each regulatory object by the regulatory authorities;
ïµ Affordable: the price of the flower colloidal gold test strip, the quality and results of the chromatographic test, and the saving of manpower and material resources, greatly reducing the cost of testing;
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