Some proper terms about HG-98 immunofluorescence analyzer

Some proper terms about the HG-98 immunofluorescence analyzer:

Immunofluorescence technique: A method in which immunological methods (antigen-antibody specific binding) and fluorescent labeling techniques are combined to study the distribution of specific protein antigens in cells. The main features of this method are: strong specificity, high sensitivity and fast speed.

Immunofluorescence measurement: After the antigen-antibody reaction, the fluorescence intensity is measured by a special instrument to estimate the concentration of the analyte.

Fluorescence: refers to the fact that a molecule or atom absorbs the energy of the excitation light, and the electron transitions from the ground state to the excited state. When it returns to the ground state, it emits energy in the form of emitted light, which is called fluorescence.

Fluorescein: An organic compound that can be used as a dye to absorb fluorescence and absorb light. It is also called a fluorescent pigment.

Excitation wavelength: means that the fluorescein is excited by this wavelength and can detect the intensity of the emitted fluorescence.

Emission wavelength: refers to the wavelength at which the fluorescein is excited by the emission wavelength to emit fluorescence intensity.

Fluorescent microspheres: In addition to fluorescein, HG-98 immunofluorescence analyzer is often used to detect reagents with fluorescent microspheres. Fluorescent microspheres act as a special functional microsphere. The fluorescent microspheres are used as immobilized carriers for different detection targets in biological sample detection. Different fluorescent microspheres correspond to different capture antibodies and recognize different antigens, thereby realizing qualitative and quantitative detection of various antigens to be tested. Moreover, fluorescent microspheres can carry many fluorescent molecules relative to fluorescein, and weaker stimuli can induce strong signals, so only a small amount of low-energy radiation can be used to generate fluorescent signals, avoiding the use of conventional large-energy excitation, and The cost is reduced without losing the sensitivity of the detection.

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