Breed name: Puffer fish, commonly known as: River dolphins, Tamba fish, scientific name: Fugn rubripes T & S (Finish Oriental dolphins) Fuugupssedommus Chu (Octopus oriental dolphins) Fugu obscurus Abe (Imbergel oriental dolphins (a) broodstock, eggs and transport From May to June each year, the brooding group swims up to the shoreline spawning group as broodstock, and the adult fish weight is in the range of 1.5-4kg, the ratio of male to female is 1:9, and the male to female ratio of 3-7kg is 1:3. The broodstock caught by the fishery method is best to be artificially inseminated within two hours after catching so that a higher fertility rate can be obtained.The red fin eastern porpoise is a one-time spawning type fish, and the spawning water temperature is about 17°C. The season of eggs is concentrated, and the period of production is very short.The puffer fish breeds eggs with stickiness and it is advisable to use eggs in plastic buckets that are not easily attached.Then add 5-10L of clarified seawater to the bowl, squeeze the eggs into the water, and then add 1 - 2 tails of the male fish, make the seawater milky blood, after stirring for 5-10min, wash the eggs successively with clean water 3-5 times, until the seawater is completely clarified. Within a few hours short-distance transport of fertilized eggs, can make the capacity for 20L plastic bag containing 10L of seawater and 1.5 million eggs Filled with 5L pure oxygen and sealed, then transported into the packing box.The long-distance transportation of 7-10h is filled with 5L of seawater and 50,000 eggs, then added with 5010-6 of streptomycin sulfate. Antibacterial propagation: Zhakou sealed packing and waiting for transport.If it takes a few days to transport, you need to inflate the bag and change the water one or two times a day, if you do not have inflated conditions, you can increase the number of water changes. The egg diameter of the fin eastern dolphins is 1.2-1.4 mm. The newly fertilized eggs are soft and harden after several hours. After a few days, the volume and weight of the fertilized eggs are about 12-15% lower than those of the first fertilization. After a few days, the fertilized eggs are Grams 600-700 are calculated because the egg membrane is opaque, it is not easy to observe the development, but after 4-5 days, the fertilized egg turns yellow or purple, the live fertilized egg is milky white and glossy. It is easy to identify through the egg membrane.Use a transparent PVC incubator, diameter 10cm, height 100cm, volume 180L, the bottom is funnel-shaped, with an egg trap in the middle.Inflate from the bottom to keep the fish eggs constantly turning.From above the incubator Inlet water and drainage pipe from the bottom up, keep the circulation, maximum daily The amount of water is 8. The incubator should be placed in a room with a illuminance of about 500 L. Each incubator can contain 5-10 million fertilized eggs, and it is generally preferable to put 200-300,000 grains in. The incubation time varies depending on the water temperature, 13-15. °C takes about 15 days, 15-17 °C takes about 10 days, generally takes about 3-4 days from the start of hatching to the end, very little hatching on the first day, hatching on the second day 70%, hatching on the third and fourth days 20-30% When separating the larvae from hatching eggs, stop inflating, the egg sinks at the bottom, and the larvae float up to the middle and upper layers, then you can scoop out all the newly hatched larvae, and then gently stir the dead eggs with small proportion of seawater and collect them on the surface. Centrally, it can be easily removed.(3) Embryo and larvae development characteristics The puffer fish eggs are not transparent, so continuous observation of embryonic development is more difficult, as described below only in the development of 18 °C -20 °C water temperature conditions. 15h after fertilization is a multicell stage, 24h after fertilization is a high blastocyst stage, 30h after fertilization is a low blastocyst period, 42h after fertilization is the original intestine stage, 3 days after fertilization embryo formation, 4 days after fertilization as the eye period, Embryos were 1/2 around egg yolk and larvae hatched 6 days after fertilization. The newly hatched larvae have a full length of 2.4-2.6mm. The yolk sac is large. There are a large number of yellow pigment cells on the surface of the oil globules. The eyes are large, the mouth concave is small, the body is round and thick, and the pectoral fins are formed. The larvae on the third day after hatching had a total length of 2.7-3.2mm, an average of 3.0mm, and the mouth had been opened, and the caliber was about 300mm. Underdeveloped jaws, yolk sac remaining 1/2. The larvae on the 5th day after hatching were 3.2mm-3.8mm in length and 3.4um in average. The yolk sac disappeared and a few survived. Has begun to eat rotifers and shellfish larvae. The larvae on the 10th day after hatching are about 5mm in length. The pectoral fins are more developed and the anal fins have differentiated. At this point larvae began to appear "drum" habits, from the performance of inhalation after the water or encountered the enemy, the abdomen expanded like a ball, began to feed Artemia larvae. After larvae on the 16th day after hatching, the larvae were about 6.5mm in length and nearly 8mm in size. When they entered the juvenile stage, the dorsal, caudal, and anal fins began to differentiate and form teeth, and a large number of small prickles appeared in front of the abdomen. At this time, the swimming speed is fast and the cluster is obvious, mainly feeding Artemia larvae. The larvae on the 25th day after hatching have a total length of 18-20mm. The fins are well-developed, yellowish, and greenish on their backs. Each side of the left and right pectoral fins has a white circle-shaped “big round spotâ€. The upper and lower jaws form two well-developed incisors. Become young fish. Feed on Artemia adults, shrimp or fish gills. (4) The fry cultivation can be carried out in a large cement pool or a small glass steel tank in the early stage of cultivation, and it can be cultivated to 5-6mm, which generally takes 10-15 days. 1 Cultivation conditions. Use 20-60m3 cement tank or 1-2m3 fiberglass tank. The inner wall of the container is based on the dark color. The illuminance is controlled at 500-1000LX. The water temperature is 15-20°C, PH7.8-8.2, salinity 28- 30%, dissolved oxygen is not less than 4mg/l, can be micro-inflated, the culture density of 20,000 / m3-30 000 / m3 early hatch larvae is appropriate. 2 bait feed. The newly hatched larvae were open the next day, the mouth cracked about 300um, can be directly fed rotifers as an open bait, rotifer feeding density of 10-20 / ml, feeding 3-6 times a day. When larvae are close to 5mm in length, they can be supplemented with Artemia larvae or copepods. 3 daily management. In the early stage, hydrostatic cultivation can be adopted, changing the water 2-3 times per day, changing the water volume every time by 1/3-1/2, and changing the water before feeding. Clear bottom 1-2 times daily. The light transmittance of the workshop should be set up shading screen adjustment. Pay attention to the observation of water quality and food intake, observation of growth, and finding problems in time to take measures. 4 out of the pool. When the larvae are full-length to 5-6mm, there will be differences among individuals, mutual disability phenomenon, and at the same time the density will influence the cultivation. Rapidly lower the water level when leaving the pond, remove the eel fry count, and move it to the rear cultivation tank. The pre-cultivation survival rate is generally around 50-60%. Late breeding 1 cultivation conditions. 20-60m3 cement pool is generally used. The water depth is about 1m. The illuminance increases with the growth of the fry. It can be directly cultivated in the outdoor open-air pool. When the indoor cultivation, the light transmittance can be increased to facilitate the increase of the water temperature and accelerate the growth of the fry. The water temperature should be increased to 20-28°C. The culture density should not be too high, with 1500-2000 tails/m3 being appropriate. 2 bait feed. Artemia larvae or copepods are mainly Artemia larvae feeding amount is 0.1-1/mL, feeding 4-6 times a day, increasing the amount of feeding and times as the fry grows, and starting when 9-10mm. Feed chilled baits such as prawn and fish oysters until they are out of the pond. In recent years, there have also been examples of using homemade bait to feed fry. In short, no matter what kind of bait is used, it is necessary to ensure adequate quality and supply in order to prevent the increase in residual food. 3 Growth and ecological characteristics. 5mm larvae body color will change with the intensity of light, and the phenomenon of "drumbling" and mutual attacks, but the residual food is not yet obvious, 7-8mm into the juvenile period, the initial formation of teeth, began to eat each other and As the growth of residual food intensifies, the attacker “drums himself until he is bitten to death. At this time, the mortality rate of fry is increased. The organs of fry above 12 mm are basically well-developed and transferred to middle and lower levels of activities and feeding. The total length is about 18 mm, and metamorphosis is Juvenile fish, basically the same shape as the fish.Into the late cultivation, the growth rate significantly accelerated, and the outdoor slightly faster than the indoor.The survival rate of this period is 35-50%. 4 daily management.Change water or water culture can be. Water 0.5-1 times, 8mm when the amount of water increased to 2 times, after feeding chilled bait, the water quality is easily contaminated, the flow of water should be cultivated and exchanged 2-4 times a day, the amount of inflation is medium, and the amount of feed to be gradually increased. Number of times, especially in the early hours of the day, feeding in a timely manner, entering the larvae period can be divided according to different specifications.Usually strengthen the water quality testing and disease prevention.Out of the pool.Usually out of the specifications for the 20-30mm.Fish at this time has been Enter young fish Stepping up, adaptability is strengthened, stability is cultivated, and it can be used for breeding and breeding in a timely manner.6 Transportation The main tools for seedling transportation are live water boats, oxygenated bags, canvas buckets, etc. In recent years, domestic use of closed type has begun. Live fish transportation vehicles and oxygen-filled bags are widely used in long-distance transportation.
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