The American bullfrog (Rana catesbeiana) has a unique ability to survive in cold conditions, especially during its tadpole stage. Tadpoles can withstand freezing temperatures as long as there is water beneath the ice. When the temperature drops below 10°C, it's crucial to prepare the pond for winter by cleaning it and ensuring it's deep enough to maintain water levels above the surface. Adding some fertilizer and feeding them appropriately can help increase their survival rate, which can reach up to 90% if properly managed.
Before the tadpoles fully develop into frogs—when they still have their tails but haven't completely shed them—they are more vulnerable to cold and environmental stress. This stage is critical, as mortality rates can be as high as 50% or more after winter or early spring. To protect them, two common methods are used: covering the ponds with rice straw or plastic films to insulate them, or building indoor pools with depths over 1 meter and using heating systems to maintain water temperatures between 0°C and 5°C.
Once the frogs are fully developed and the temperature drops below 5°C, they enter hibernation. There are two main methods for wintering: one involves leaving them in water, where they burrow into 20–30 cm of mud at the bottom of a sunny, sheltered pond. The other method is land hibernation, where loose soil or small holes (about 13–15 cm in diameter and 1 meter deep) are prepared around the pond. These should remain moist but not flooded, and covered with thick layers of soft weeds for protection. It's important to guard against predators like rats, snakes, and cats during this time.
For the Chinese sturgeon (Acipenser sinensis), the survival rate during winter drops significantly for young fish weighing less than 4 grams, with mortality rates reaching 50%–60%. Artificial winter protection is essential. One approach is to use indoor breeding ponds, either for deep or shallow water hibernation. In deep water, the pond should be drained first, with 20–30 cm of sediment and 5–10 cm of water added. Then, 100–150 juvenile sturgeons per cubic meter are placed inside. When the water temperature falls below 10°C, they will naturally burrow into the sand. The pond is then refilled, and a straw curtain on top with 20 cm of insulation helps keep the temperature above 0°C.
In shallow water, the pond floor should be covered with sand, and 30–40 cm of straw should be laid to allow the young sturgeons to hide in the straw as winter ends. By April of the following year, when the temperature reaches 18°C, the water is partially drained, leaving only 5–10 cm, or the straw is removed and fresh water is added.
For juvenile and adult clams, wintering depends on their size. Indoor protection is ideal, following similar methods used for young clams. If outdoor ponds are used, they should be located in sunny, sheltered areas with quiet surroundings. The pond should have more than 30 cm of sediment, and the water level should be deepened to at least 1 meter under the ice. Covering the pond with plastic film improves insulation and increases survival chances.
Applying Bacillus mucilaginosus to soil can be a game-changer for farmers looking to enhance soil fertility and crop productivity sustainably. By understanding the bacterium’s benefits and following the outlined application methods, you can create an optimal environment for your plants to thrive. Regular use of Bacillus mucilaginosus not only improves nutrient availability but also promotes healthier soil, leading to better long-term agricultural outcomes.
Whether you are a small-scale gardener or a large-scale farmer, incorporating Bacillus mucilaginosus into your soil management practices can lead to healthier crops and more bountiful harvests. By following the tips and techniques shared in this blog, you can maximize the benefits of this remarkable bacterium and take a significant step towards more sustainable farming.

Bacillus Mucilaginosus
Biodep Biotechnology Co. ,Ltd. , https://www.biodep.com